The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit

Structural basis for hygromycin B inhibition of protein biosynthesis.

Aminoglycosides are one of the most widely used and clinically important classes of antibiotics that target the ribosome. Hygromycin B is an atypical aminoglycoside antibiotic with unique structural and functional properties. Here we describe the structure of the intact Escherichia coli 70S ribosome in complex with hygromycin B. The antibiotic binds to the mRNA decoding center in the small (30S...

Specificity in the mechanism of action of antibiotic inhibitors of protein and nucleic acid synthesis.

Antibiotics that interfere with protein synthesis have been distinguished by their effect on procaryotic or euraryotic cells and extracts, or both; by their binding or action on the smaller or larger ribosome subunit; and by their action on the Por A-site of the ribosomal-mRNA complex. Sparsomycin, chioramphenicol. gougerotin and amicetin all inhibit peptide bond formation and interact with the...

SnapShot: Antibiotic Inhibition of Protein Synthesis I

Elongation (translocation) Most aminoglycoside antibiotics induce translational misreading by promoting binding of near-cognate tRNAs, but the biological effect is probably due to inhibition of the translocation reaction and promotion of back-translocation. Edeine Edeine A Initiation (fMet-tRNA binding) Edeine prevents binding of the initiator tRNA to the 30S subunit in an mRNA-dependent manner...

Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coli.

The aminoglycoside antibiotic hygromycin B was examined in Escherichia coli cells for inhibitory effects on translation and ribosomal-subunit formation. Pulse-chase labeling experiments were performed, which verified lower rates of ribosomal-subunit synthesis in drug-treated cells. Hygromycin B exhibited a concentration-dependent inhibitory effect on viable-cell numbers, growth rate, protein sy...

Antibiotics that Inhibit 30S or 50S Ribosomal Subunit Formation: Hygromycin B, Quinupristin-Dalfopristin and XRP 2868